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Basic Acclimatization Skill

On this page, you will find all topics related to Basic Acclimatization Skill! Here Every Major Topic Includes Sub-Major Topics. Find the topic you want to learn!   Introduction-  What is BAS? Why it is important? Introduction to Accommodation Management- Telephone Handling Techniques,  Organizing of Rooms,  Cleaning Equipment and Uses,  Bed Making Process ,  Fundamentals of Cooking- Uses of Basic Cooking equipment,  Uses of Fire and Fuel,  Different Cuts of Vegetables,  Uses of Herbs and Spices,  Regional Food Habits. Food and Beverages Skills-  Introduction to Catering Industry  Types of Menu and Food & Beverages  Identification of Equipment  Chef Knife Table Manners  Customer Handling Skills  Travel Management   Travel or Documentation  Application for Passport and Visa  Tourism Product (UNESCO Sites)  Types of Logistics  Basic Hospitality Skills-  Various Egg Preparation...

Lambda Phage Vector

Lambda (λ) vectors, also known as lambda phage vectors, are widely used tools in molecular biology for cloning large DNA fragments. Here's an explanation of λ vectors: Origin :  Lambda vectors are derived from the lambda bacteriophage, a virus that infects bacteria, particularly Escherichia coli (E. coli). Structure :  Lambda phage has a linear double-stranded DNA genome with cohesive ends (Sticky Ends), also known as cos sites, at each end.  Lambda vectors are modified versions of the lambda phage genome, where the non-essential genes are replaced with DNA sequences required for cloning and propagation in bacterial hosts. Insertion Capacity :  Lambda vectors have a large cloning capacity, capable of accommodating DNA fragments ranging from approximately 8 to 20 kilobases (kb) in size.  This makes them particularly useful for cloning relatively large DNA fragments compared to other vectors like plasmids. Cosmid Vectors :  One common type of λ vector is the ...

Construction and screening of genomic DNA library and cDNA library

  Introduction DNA libraries are indeed collections of DNA fragments that have been cloned into vectors, which allow researchers to isolate and study specific genes or genomic regions.  Isolation of Genes : With approximately 25,000 genes in the human genome spread across 3 billion base pairs of DNA, isolating specific genes of interest is crucial for research. DNA Library Construction : One method for isolating genes involves constructing a DNA library. This involves cloning DNA fragments into vectors, such as plasmids or bacteriophages, to create a collection of DNA molecules representing the entire genome or a subset of it. Cloning : When a gene is identified and copied, it is said to have been "cloned." This process involves inserting the gene of interest into a vector, which serves as a carrier to replicate and express the gene. Definition Definition of "Library" : The term "library" can refer either to a population of organisms, each carrying a DNA m...