Subjects Are...........
- Engineering Mathematics
- General Biology
- Genetics, Cellular and Molecular Biology
- Fundamentals of Biological Engineering
- Bioprocess Engineering and Process Biotechnology
- Plant, Animal and Microbial Biotechnology
- Recombinant DNA technology and Other Tools in Biotechnology
- UNIT 1
- Biomolecules-structure and functions;
- Biological membranes, structure, action potential and transport processes;
- Enzymes- classification, kinetics and mechanism of action;
- Basic concepts and designs of metabolism (carbohydrates, lipids, amino acids and nucleic acids) photosynthesis, respiration and electron transport chain;
- Bioenergetics.
- UNIT 2
- Virus: Structure and classification;
- Microbial classification and diversity (bacterial, algal and fungal);
- Methods in microbiology;
- Microbial growth and nutrition;
- Aerobic and anaerobic respiration;
- Nitrogen fixation;
- Microbial diseases and host-pathogen interaction.
- UNIT 4
- Molecular structure of genes and chromosomes
- Mutation and Mutagenesis
- Nucleic Acid Replication, Transcription, Translation, and Regulatory Mechanisms
- UNIT 7
- Restriction and modification enzymes;
- Vectors; plasmid, bacteriophage and other viral vectors, cosmids, Ti plasmid, yeast artificial chromosome;
- Mammalian and plant expression vectors;
- cDNA and genomic DNA library;
- Gene isolation, cloning and expression;
- Transposons and gene targeting;
- DNA labeling;
- DNA sequencing;
- Polymerase chain reactions;
- DNA fingerprinting;
- Southern and northern blotting;
- In- situ hybridization;
- RAPD, RFLP;
- Site-directed mutagenesis;
- Gene transfer technologies;
- Gene therapy.