On this page, you will find all topics related to Genetic Engineering!
Here Every Major Topic Includes Sub-Major Topics. Find the topic you want to learn!
- Introduction to Genetic Engineering
- Definition,
- History and scope.
- Restriction enzymes: definition, characteristics and uses.
- Types of Restriction Enzymes and their functions
- Cloning and cloning vectors
- Clear your concept about vectors by clicking here
- Vectors and their characteristics
- Molecular Cloning
- Types of Cloning Vectors
- Plasmid vectors,
- Example of Plasmid Vectors
- λ vectors.
- Construction and screening of genomic DNA library and cDNA library
- Molecular detection techniques-
- Southern, Northern and Western hybridization,
- Polymerase chain reaction (PCR),
- DNA Sequencing
- Restriction Fragment Length Polymorphism(RFLP),
- Random Amplified Polymorphic DNA (RAPD),
- DNA fingerprinting.
- Di-deoxy Nucleic acid sequencing: Di-deoxy and chemical sequencing methods.
- Practical application of Recombinant DNA technology
- Engineering of bacteria,
- Genetically engineered biopharmaceuticals (insulin and growth hormones),
- Ti plasmid in plant biotechnology,
- Vaccine production
- Molecular detection of disease
- DNA Ligase