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Shake Flask Experiment and Bioreactor Explanation

Shake Flask Experiment and Bioreactor Explanation

Bioreactor

  • A bioreactor is a device in which organisms are cultivated and motivated to form the desired product.

  • It is a containment system designed to provide a controlled environment for the optimal growth and activity of organisms.

  • The word fermentation originates from the Latin word fervere, which literally means "to boil."

Solid State Fermentation (SSF)

  • There are certain fermentation processes that do not involve liquid medium. For such biotechnology processes, the growth of microorganisms is carried out on solid substances in the complete absence of free water.

  • The most commonly used solid substrates for SSF are:

    • Cereal grains

    • Wheat bran

    • Sawdust

    • Wood shavings

    • Several other plant and natural materials

  • These substrates are polymeric in nature, insoluble in water, and contain a concentrated source of nutrients required for the growth of microorganisms.

  • Solid state fermentation has been very popular for the production of fermented foods such as:

    • Idli

    • Dosa

    • Dhokla

    • Bread

    • Beverages

    • Fermented fish

    • Meat

    • Yogurt

    • Cheese

    • Pickles


Bioreactor for Solid State Fermentation

  • The types of bioreactors used in SSF are:

    • Drum reactor

    • Forced aeration reactor


  • Advantages of SSF:

    • Solid substrate fermentation employs simple, natural solid media.

    • Low technology requirements and low energy expenditure.

    • No need for sterilization.

    • Less microbial contamination and easy downstream processing

    • Bioreactor design and effluent treatment are very simple

    • Yield of the product is reasonably high


Shake Flusk Experiment

  • Imagine you have a special bottle (a flask) with a nutrient-rich liquid inside. You want to grow some tiny organisms, like bacteria or yeast, in this liquid.

  • To help these organisms grow well, you need to provide them with oxygen. One way to do this is by shaking the flask. As you shake it, the liquid sloshes around, and oxygen from the air mixes into the liquid.

  • This shaking process is called a "shake flask experiment." It's a simple but effective way to grow microorganisms in a laboratory setting.

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