USING OF PH INDICATOR DURING ANIMAL CELL CULTURE:

PH Indicator

• Neutral (Suitable): Red (pH ~7)

• Acidic: Yellow (pH < 7)

• Basic: Purple (pH > 7)

Media Use in Animal Cell Culture:

• The media used in animal cell culture provides essential nutrients, growth factors, and environmental conditions necessary for cell survival and growth.

pH Indicator:

• Phenol Red is the pH indicator commonly used in animal cell culture media.

• Colour of the Media: Typically, the media appears red due to the presence of phenol red.

Function of Phenol Red:

• Phenol red helps monitor the pH of the culture medium, which is crucial for maintaining optimal conditions for cell growth.

• Optimal pH: The normal or optimal pH for animal cell culture is around 7, which is considered neutral.

pH Detection:

• The pH indicator will change colour if there is any fluctuation in the pH of the media:

• Neutral (Suitable): Red (pH ~7)

• Acidic: Yellow (pH < 7)

• Basic: Purple (pH > 7)

pH Fluctuation

• A pH fluctuation of only 0.2 units is generally allowed. Significant deviations from the optimal pH can adversely affect cell health and growth.